Search results for "DNA Packaging"
showing 5 items of 5 documents
Nucleotide's bilinear indices: Novel bio-macromolecular descriptors for bioinformatics studies of nucleic acids. I. Prediction of paromomycin's affin…
2009
A new set of nucleotide-based bio-macromolecular descriptors are presented. This novel approach to bio-macromolecular design from a linear algebra point of view is relevant to nucleic acids quantitative structure-activity relationship (QSAR) studies. These bio-macromolecular indices are based on the calculus of bilinear maps on Re(n)[b(mk)(x (m),y (m)):Re(n) x Re(n)--Re] in canonical basis. Nucleic acid's bilinear indices are calculated from kth power of non-stochastic and stochastic nucleotide's graph-theoretic electronic-contact matrices, M(m)(k) and (s)M(m)(k), respectively. That is to say, the kth non-stochastic and stochastic nucleic acid's bilinear indices are calculated using M(m)(k)…
Efficient DNA Packaging of Bacteriophage PRD1 Requires the Unique Vertex Protein P6
2007
ABSTRACT The assembly of bacteriophage PRD1 proceeds via formation of empty procapsids containing an internal lipid membrane, into which the linear double-stranded DNA genome is subsequently packaged. The packaging ATPase P9 and other putative packaging proteins have been shown to be located at a unique vertex of the PRD1 capsid. Here, we describe the isolation and characterization of a suppressor-sensitive PRD1 mutant deficient in the unique vertex protein P6. Protein P6 was found to be an essential part of the PRD1 packaging machinery; its absence leads to greatly reduced packaging efficiency. Lack of P6 was not found to affect particle assembly, because in the P6-deficient mutant infecti…
The Complex Regulatory Role of Cytomegalovirus Nuclear Egress Protein pUL50 in the Production of Infectious Virus
2021
The regulation of the nucleocytoplasmic release of herpesviral capsids is defined by the process of nuclear egress. Due to their large size, nuclear capsids are unable to traverse via nuclear pores, so that herpesviruses evolved to develop a vesicular transport pathway mediating their transition through both leaflets of the nuclear membrane. This process involves regulatory proteins, which support the local distortion of the nuclear envelope. For human cytomegalovirus (HCMV), the nuclear egress complex (NEC) is determined by the pUL50-pUL53 core that initiates multicomponent assembly with NEC-associated proteins and capsids. Hereby, pUL50 serves as a multi-interacting determinant that recru…
Der Einfluss kondensierter Phosphate auf die Eisen-Resorption
1959
Es sollte untersucht werden, ob die kondensierten Phosphate Tetranatriumpyrophosphat, Natriumtripolyphosphat, Hexametaphosphat, Kaliummetaphosphat und Plasmal die Eisenresorption beeintrachtigen. Als Masstab fur diesselbe galten die Hamoglobin- und Erythrocytenregeneration und die Gewichtszunahme anamischer Ratten. Die Anamie wurde durch Phenylhydrazinhydrochlorid-Injektionen bzw. eine eisenarme Ernahrung (Milchdiat) hervorgerufen. Bei den herrschenden Versuchsbedingungen konnte ein nachteiliger Einflus der getesteten Substanzen auf die Eisenresorption nicht nachgewiesen werden. Auf Grund dieser Versuche und allgemeiner Uberlegungen wurde geschlossen, das dies uberhaupt unter physiologische…
A Headful of T4 Coliphage DNA Packaged to Fasces-Like Globules in Fractal Models
1998
We present a new model for T4 DNA packaging based on fractal considerations. The proposed model is based on electron microscopic observations of spread and packaged DNA. The model takes into account enzymatically and unidirectionally driven packaging, and quick release of the DNA during infection. We also consider the different biochemical reactions of the packaging process.